3D estimation of synaptic vesicle distributions in serial section transmission electron microscopy

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Standard

3D estimation of synaptic vesicle distributions in serial section transmission electron microscopy. / Khanmohammadi, Mahdieh; Darkner, Sune; Nava, Nicoletta; Nyengaard, Jens Randel; Wegener, G; Popoli, M.; Sporring, Jon.

I: Journal of Microscopy, Bind 265, Nr. 1, 2017, s. 101–110.

Publikation: Bidrag til tidsskriftTidsskriftartikelfagfællebedømt

Harvard

Khanmohammadi, M, Darkner, S, Nava, N, Nyengaard, JR, Wegener, G, Popoli, M & Sporring, J 2017, '3D estimation of synaptic vesicle distributions in serial section transmission electron microscopy', Journal of Microscopy, bind 265, nr. 1, s. 101–110. https://doi.org/10.1111/jmi.12468

APA

Khanmohammadi, M., Darkner, S., Nava, N., Nyengaard, J. R., Wegener, G., Popoli, M., & Sporring, J. (2017). 3D estimation of synaptic vesicle distributions in serial section transmission electron microscopy. Journal of Microscopy, 265(1), 101–110. https://doi.org/10.1111/jmi.12468

Vancouver

Khanmohammadi M, Darkner S, Nava N, Nyengaard JR, Wegener G, Popoli M o.a. 3D estimation of synaptic vesicle distributions in serial section transmission electron microscopy. Journal of Microscopy. 2017;265(1):101–110. https://doi.org/10.1111/jmi.12468

Author

Khanmohammadi, Mahdieh ; Darkner, Sune ; Nava, Nicoletta ; Nyengaard, Jens Randel ; Wegener, G ; Popoli, M. ; Sporring, Jon. / 3D estimation of synaptic vesicle distributions in serial section transmission electron microscopy. I: Journal of Microscopy. 2017 ; Bind 265, Nr. 1. s. 101–110.

Bibtex

@article{4788205ee4134c01ba3e766a03a73c4d,
title = "3D estimation of synaptic vesicle distributions in serial section transmission electron microscopy",
abstract = "Behavioural stress has shown to strongly affect neurotransmission within the neocortex. In this study, we analysed the effect of an acute stress model on density and distribution of neurotransmitter-containing vesicles within medial prefrontal cortex. Serial section transmission electron microscopy was employed to compare two groups of male rats: (1) rats subjected to foot-shock stress and (2) rats with sham stress as control group. Two-dimensional (2D) density measures are common in microscopic images and are estimated by following a 2D path in-section. However, this method ignores the slant of the active zone and thickness of the section. In fact, the active zone is a surface in three-dimension (3D) and the 2D measures do not accurately reflect the geometric configuration unless the active zone is perpendicular to the sectioning angle. We investigated synaptic vesicle density as a function of distance from the active zone in 3D. We reconstructed a 3D dataset by estimating the thickness of all sections and by registering all the image sections into a common coordinate system. Finally, we estimated the density as the average number of vesicles per area and volume and modelled the synaptic vesicle distribution by fitting a one-dimensional parametrized distribution that took into account the location uncertainty due to section thickness. Our results showed a clear structural difference in synaptic vesicle density and distribution between stressed and control group with improved separation by 3D measures in comparison to the 2D measures. Our results showed that acute foot-shock stress exposure significantly affected both the spatial distribution and density of the synaptic vesicles within the presynaptic terminal.",
author = "Mahdieh Khanmohammadi and Sune Darkner and Nicoletta Nava and Nyengaard, {Jens Randel} and G Wegener and M. Popoli and Jon Sporring",
year = "2017",
doi = "10.1111/jmi.12468",
language = "English",
volume = "265",
pages = "101–110",
journal = "Journal of Microscopy",
issn = "0022-2720",
publisher = "Wiley-Blackwell",
number = "1",
note = "European Congress of Stereology and Image Analysis, ECS ; Conference date: 08-07-2013 Through 09-07-2013",

}

RIS

TY - JOUR

T1 - 3D estimation of synaptic vesicle distributions in serial section transmission electron microscopy

AU - Khanmohammadi, Mahdieh

AU - Darkner, Sune

AU - Nava, Nicoletta

AU - Nyengaard, Jens Randel

AU - Wegener, G

AU - Popoli, M.

AU - Sporring, Jon

N1 - Conference code: 11

PY - 2017

Y1 - 2017

N2 - Behavioural stress has shown to strongly affect neurotransmission within the neocortex. In this study, we analysed the effect of an acute stress model on density and distribution of neurotransmitter-containing vesicles within medial prefrontal cortex. Serial section transmission electron microscopy was employed to compare two groups of male rats: (1) rats subjected to foot-shock stress and (2) rats with sham stress as control group. Two-dimensional (2D) density measures are common in microscopic images and are estimated by following a 2D path in-section. However, this method ignores the slant of the active zone and thickness of the section. In fact, the active zone is a surface in three-dimension (3D) and the 2D measures do not accurately reflect the geometric configuration unless the active zone is perpendicular to the sectioning angle. We investigated synaptic vesicle density as a function of distance from the active zone in 3D. We reconstructed a 3D dataset by estimating the thickness of all sections and by registering all the image sections into a common coordinate system. Finally, we estimated the density as the average number of vesicles per area and volume and modelled the synaptic vesicle distribution by fitting a one-dimensional parametrized distribution that took into account the location uncertainty due to section thickness. Our results showed a clear structural difference in synaptic vesicle density and distribution between stressed and control group with improved separation by 3D measures in comparison to the 2D measures. Our results showed that acute foot-shock stress exposure significantly affected both the spatial distribution and density of the synaptic vesicles within the presynaptic terminal.

AB - Behavioural stress has shown to strongly affect neurotransmission within the neocortex. In this study, we analysed the effect of an acute stress model on density and distribution of neurotransmitter-containing vesicles within medial prefrontal cortex. Serial section transmission electron microscopy was employed to compare two groups of male rats: (1) rats subjected to foot-shock stress and (2) rats with sham stress as control group. Two-dimensional (2D) density measures are common in microscopic images and are estimated by following a 2D path in-section. However, this method ignores the slant of the active zone and thickness of the section. In fact, the active zone is a surface in three-dimension (3D) and the 2D measures do not accurately reflect the geometric configuration unless the active zone is perpendicular to the sectioning angle. We investigated synaptic vesicle density as a function of distance from the active zone in 3D. We reconstructed a 3D dataset by estimating the thickness of all sections and by registering all the image sections into a common coordinate system. Finally, we estimated the density as the average number of vesicles per area and volume and modelled the synaptic vesicle distribution by fitting a one-dimensional parametrized distribution that took into account the location uncertainty due to section thickness. Our results showed a clear structural difference in synaptic vesicle density and distribution between stressed and control group with improved separation by 3D measures in comparison to the 2D measures. Our results showed that acute foot-shock stress exposure significantly affected both the spatial distribution and density of the synaptic vesicles within the presynaptic terminal.

U2 - 10.1111/jmi.12468

DO - 10.1111/jmi.12468

M3 - Journal article

VL - 265

SP - 101

EP - 110

JO - Journal of Microscopy

JF - Journal of Microscopy

SN - 0022-2720

IS - 1

T2 - European Congress of Stereology and Image Analysis

Y2 - 8 July 2013 through 9 July 2013

ER -

ID: 379982466