Cross-reactive metal ion sensor array in a micro titer plate format

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Standard

Cross-reactive metal ion sensor array in a micro titer plate format. / Mayr, Torsten; Igel, Christian; Liebsch, Gregor; Klimant, Ingo; Wolfbeis, Otto S.

I: Analytical Chemistry, Bind 75, Nr. 17, 2003, s. 4389-4396.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningfagfællebedømt

Harvard

Mayr, T, Igel, C, Liebsch, G, Klimant, I & Wolfbeis, OS 2003, 'Cross-reactive metal ion sensor array in a micro titer plate format', Analytical Chemistry, bind 75, nr. 17, s. 4389-4396. https://doi.org/10.1021/ac020774t

APA

Mayr, T., Igel, C., Liebsch, G., Klimant, I., & Wolfbeis, O. S. (2003). Cross-reactive metal ion sensor array in a micro titer plate format. Analytical Chemistry, 75(17), 4389-4396. https://doi.org/10.1021/ac020774t

Vancouver

Mayr T, Igel C, Liebsch G, Klimant I, Wolfbeis OS. Cross-reactive metal ion sensor array in a micro titer plate format. Analytical Chemistry. 2003;75(17):4389-4396. https://doi.org/10.1021/ac020774t

Author

Mayr, Torsten ; Igel, Christian ; Liebsch, Gregor ; Klimant, Ingo ; Wolfbeis, Otto S. / Cross-reactive metal ion sensor array in a micro titer plate format. I: Analytical Chemistry. 2003 ; Bind 75, Nr. 17. s. 4389-4396.

Bibtex

@article{1092e4e3e87e4995a71d740db06831a2,
title = "Cross-reactive metal ion sensor array in a micro titer plate format",
abstract = "A cross-reactive array in a micro titer plate (MTP) format is described that is based on a versatile and highly flexible scheme. It makes use of rather unspecific metal ions probes having almost identical fluorescence spectra, thus enabling (a) interrogation at identical analytical wavelengths, and (b) imaging of the probes contained in the wells of the MTP using a CCD camera and an array of blue-light-emitting diodes as a light source. The unselective response of the indicators in the presence of mixtures of five divalent cations generates a characteristic pattern that was analyzed by chemometric tools. The fluorescence intensity of the indicators was transferred into a time-dependent parameter applying a scheme called dual lifetime referencing. In this method, the fluorescence decay profile of the indicator is referenced against the phosphorescence of an inert reference dye added to the system. The intrinsically referenced measurements also were performed using blue LEDs as light sources and a CCD camera without intensifiers as the detector. The best performance was observed if each well was excited by a single LED. The assembly allows the detection of dye concentrations in the nanomoles-per-liter range without amplification and the acquisition of 96 wells simultaneously. The pictures obtained form the basis for evaluation by pattern recognition algorithms. Support vector machines are capable of predicting the presence of significant concentrations of metal ions with high accuracy.",
author = "Torsten Mayr and Christian Igel and Gregor Liebsch and Ingo Klimant and Wolfbeis, {Otto S}",
year = "2003",
doi = "10.1021/ac020774t",
language = "English",
volume = "75",
pages = "4389--4396",
journal = "Analytical Chemistry",
issn = "0003-2700",
publisher = "American Chemical Society",
number = "17",

}

RIS

TY - JOUR

T1 - Cross-reactive metal ion sensor array in a micro titer plate format

AU - Mayr, Torsten

AU - Igel, Christian

AU - Liebsch, Gregor

AU - Klimant, Ingo

AU - Wolfbeis, Otto S

PY - 2003

Y1 - 2003

N2 - A cross-reactive array in a micro titer plate (MTP) format is described that is based on a versatile and highly flexible scheme. It makes use of rather unspecific metal ions probes having almost identical fluorescence spectra, thus enabling (a) interrogation at identical analytical wavelengths, and (b) imaging of the probes contained in the wells of the MTP using a CCD camera and an array of blue-light-emitting diodes as a light source. The unselective response of the indicators in the presence of mixtures of five divalent cations generates a characteristic pattern that was analyzed by chemometric tools. The fluorescence intensity of the indicators was transferred into a time-dependent parameter applying a scheme called dual lifetime referencing. In this method, the fluorescence decay profile of the indicator is referenced against the phosphorescence of an inert reference dye added to the system. The intrinsically referenced measurements also were performed using blue LEDs as light sources and a CCD camera without intensifiers as the detector. The best performance was observed if each well was excited by a single LED. The assembly allows the detection of dye concentrations in the nanomoles-per-liter range without amplification and the acquisition of 96 wells simultaneously. The pictures obtained form the basis for evaluation by pattern recognition algorithms. Support vector machines are capable of predicting the presence of significant concentrations of metal ions with high accuracy.

AB - A cross-reactive array in a micro titer plate (MTP) format is described that is based on a versatile and highly flexible scheme. It makes use of rather unspecific metal ions probes having almost identical fluorescence spectra, thus enabling (a) interrogation at identical analytical wavelengths, and (b) imaging of the probes contained in the wells of the MTP using a CCD camera and an array of blue-light-emitting diodes as a light source. The unselective response of the indicators in the presence of mixtures of five divalent cations generates a characteristic pattern that was analyzed by chemometric tools. The fluorescence intensity of the indicators was transferred into a time-dependent parameter applying a scheme called dual lifetime referencing. In this method, the fluorescence decay profile of the indicator is referenced against the phosphorescence of an inert reference dye added to the system. The intrinsically referenced measurements also were performed using blue LEDs as light sources and a CCD camera without intensifiers as the detector. The best performance was observed if each well was excited by a single LED. The assembly allows the detection of dye concentrations in the nanomoles-per-liter range without amplification and the acquisition of 96 wells simultaneously. The pictures obtained form the basis for evaluation by pattern recognition algorithms. Support vector machines are capable of predicting the presence of significant concentrations of metal ions with high accuracy.

U2 - 10.1021/ac020774t

DO - 10.1021/ac020774t

M3 - Journal article

C2 - 14632041

VL - 75

SP - 4389

EP - 4396

JO - Analytical Chemistry

JF - Analytical Chemistry

SN - 0003-2700

IS - 17

ER -

ID: 32645779