Short chain fatty acids and bile acids in human faeces are associated with the intestinal cholesterol conversion status
Research output: Contribution to journal › Journal article › Research › peer-review
Standard
Short chain fatty acids and bile acids in human faeces are associated with the intestinal cholesterol conversion status. / Matysik, Silke; Krautbauer, Sabrina; Liebisch, Gerhard; Schött, Hans-Frieder; Kjølbæk, Louise; Astrup, Arne; Blachier, Francois; Beaumont, Martin; Nieuwdorp, Max; Hartstra, Annick; Rampelli, Simone; Pagotto, Uberto; Iozzo, Patricia.
In: British Journal of Pharmacology, Vol. 178, No. 16, 2021, p. 3342-3353.Research output: Contribution to journal › Journal article › Research › peer-review
Harvard
APA
Vancouver
Author
Bibtex
}
RIS
TY - JOUR
T1 - Short chain fatty acids and bile acids in human faeces are associated with the intestinal cholesterol conversion status
AU - Matysik, Silke
AU - Krautbauer, Sabrina
AU - Liebisch, Gerhard
AU - Schött, Hans-Frieder
AU - Kjølbæk, Louise
AU - Astrup, Arne
AU - Blachier, Francois
AU - Beaumont, Martin
AU - Nieuwdorp, Max
AU - Hartstra, Annick
AU - Rampelli, Simone
AU - Pagotto, Uberto
AU - Iozzo, Patricia
N1 - This article is protected by copyright. All rights reserved.
PY - 2021
Y1 - 2021
N2 - Background and purpose: The analysis of human faecal metabolites can provide an insight into metabolic interactions between gut microbiota and host organism. The creation of metabolic profiles in faeces has received little attention until now and reference values, especially in the context of dietary and therapeutic interventions, are missing. Exposure to xenobiotics significantly affects the physiology of the microbiome and microbiota manipulation as well as short chain fatty acids administration have been proposed as treatment targets for several diseases. The aim of the present study is to give concomitant concentration ranges of faecal sterol species, bile acids and short chain fatty acids based on a large cohort.Experimental approach: Sterol species, bile acids and short chain fatty acids in human faeces from 165 study participants were quantified by LC-MS/MS. For standardization, we refer all values to dry weight of faeces. Based on the individual intestinal sterol conversion we classified participants into low and high converters according to their coprostanol/cholesterol ratio.Key results: Low converters excrete more straight chain fatty acids and bile acids than high converters. 5th , 95th percentile and median of bile acids and short chain fatty acids were calculated for both groups.Conclusion and implications: We give concentration ranges for 16 faecal metabolites that can serve as reference values. Patient stratification into high or low sterol converter groups is associated with significant differences in faecal metabolites with biological activities. Such stratification should then allow assessing faecal metabolites better before therapeutic interventions.
AB - Background and purpose: The analysis of human faecal metabolites can provide an insight into metabolic interactions between gut microbiota and host organism. The creation of metabolic profiles in faeces has received little attention until now and reference values, especially in the context of dietary and therapeutic interventions, are missing. Exposure to xenobiotics significantly affects the physiology of the microbiome and microbiota manipulation as well as short chain fatty acids administration have been proposed as treatment targets for several diseases. The aim of the present study is to give concomitant concentration ranges of faecal sterol species, bile acids and short chain fatty acids based on a large cohort.Experimental approach: Sterol species, bile acids and short chain fatty acids in human faeces from 165 study participants were quantified by LC-MS/MS. For standardization, we refer all values to dry weight of faeces. Based on the individual intestinal sterol conversion we classified participants into low and high converters according to their coprostanol/cholesterol ratio.Key results: Low converters excrete more straight chain fatty acids and bile acids than high converters. 5th , 95th percentile and median of bile acids and short chain fatty acids were calculated for both groups.Conclusion and implications: We give concentration ranges for 16 faecal metabolites that can serve as reference values. Patient stratification into high or low sterol converter groups is associated with significant differences in faecal metabolites with biological activities. Such stratification should then allow assessing faecal metabolites better before therapeutic interventions.
KW - Faculty of Science
KW - Bile acids
KW - Faecal short chain fatty acids
KW - Sterols
U2 - 10.1111/bph.15440
DO - 10.1111/bph.15440
M3 - Journal article
C2 - 33751575
VL - 178
SP - 3342
EP - 3353
JO - British Journal of Pharmacology
JF - British Journal of Pharmacology
SN - 0007-1188
IS - 16
ER -
ID: 258890251