Cellular 3D-reconstruction and analysis in the human cerebral cortex using automatic serial sections

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Cellular 3D-reconstruction and analysis in the human cerebral cortex using automatic serial sections. / Larsen, Nick Y.; Li, Xixia; Tan, Xueke; Ji, Gang; Lin, Jing; Rajkowska, Grazyna; Møller, Jesper; Vihrs, Ninna; Sporring, Jon; Sun, Fei; Nyengaard, Jens R.

In: Communications Biology , Vol. 4, No. 1, 1030, 12.2021.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Larsen, NY, Li, X, Tan, X, Ji, G, Lin, J, Rajkowska, G, Møller, J, Vihrs, N, Sporring, J, Sun, F & Nyengaard, JR 2021, 'Cellular 3D-reconstruction and analysis in the human cerebral cortex using automatic serial sections', Communications Biology , vol. 4, no. 1, 1030. https://doi.org/10.1038/s42003-021-02548-6

APA

Larsen, N. Y., Li, X., Tan, X., Ji, G., Lin, J., Rajkowska, G., Møller, J., Vihrs, N., Sporring, J., Sun, F., & Nyengaard, J. R. (2021). Cellular 3D-reconstruction and analysis in the human cerebral cortex using automatic serial sections. Communications Biology , 4(1), [1030]. https://doi.org/10.1038/s42003-021-02548-6

Vancouver

Larsen NY, Li X, Tan X, Ji G, Lin J, Rajkowska G et al. Cellular 3D-reconstruction and analysis in the human cerebral cortex using automatic serial sections. Communications Biology . 2021 Dec;4(1). 1030. https://doi.org/10.1038/s42003-021-02548-6

Author

Larsen, Nick Y. ; Li, Xixia ; Tan, Xueke ; Ji, Gang ; Lin, Jing ; Rajkowska, Grazyna ; Møller, Jesper ; Vihrs, Ninna ; Sporring, Jon ; Sun, Fei ; Nyengaard, Jens R. / Cellular 3D-reconstruction and analysis in the human cerebral cortex using automatic serial sections. In: Communications Biology . 2021 ; Vol. 4, No. 1.

Bibtex

@article{f5b1b9419b35477082aa625b6aed8e7c,
title = "Cellular 3D-reconstruction and analysis in the human cerebral cortex using automatic serial sections",
abstract = "Techniques involving three-dimensional (3D) tissue structure reconstruction and analysis provide a better understanding of changes in molecules and function. We have developed AutoCUTS-LM, an automated system that allows the latest advances in 3D tissue reconstruction and cellular analysis developments using light microscopy on various tissues, including archived tissue. The workflow in this paper involved advanced tissue sampling methods of the human cerebral cortex, an automated serial section collection system, digital tissue library, cell detection using convolution neural network, 3D cell reconstruction, and advanced analysis. Our results demonstrated the detailed structure of pyramidal cells (number, volume, diameter, sphericity and orientation) and their 3D spatial organization are arranged in a columnar structure. The pipeline of these combined techniques provides a detailed analysis of tissues and cells in biology and pathology.",
author = "Larsen, {Nick Y.} and Xixia Li and Xueke Tan and Gang Ji and Jing Lin and Grazyna Rajkowska and Jesper M{\o}ller and Ninna Vihrs and Jon Sporring and Fei Sun and Nyengaard, {Jens R.}",
note = "Publisher Copyright: {\textcopyright} 2021, The Author(s).",
year = "2021",
month = dec,
doi = "10.1038/s42003-021-02548-6",
language = "English",
volume = "4",
journal = "Communications Biology",
issn = "2399-3642",
publisher = "nature publishing group",
number = "1",

}

RIS

TY - JOUR

T1 - Cellular 3D-reconstruction and analysis in the human cerebral cortex using automatic serial sections

AU - Larsen, Nick Y.

AU - Li, Xixia

AU - Tan, Xueke

AU - Ji, Gang

AU - Lin, Jing

AU - Rajkowska, Grazyna

AU - Møller, Jesper

AU - Vihrs, Ninna

AU - Sporring, Jon

AU - Sun, Fei

AU - Nyengaard, Jens R.

N1 - Publisher Copyright: © 2021, The Author(s).

PY - 2021/12

Y1 - 2021/12

N2 - Techniques involving three-dimensional (3D) tissue structure reconstruction and analysis provide a better understanding of changes in molecules and function. We have developed AutoCUTS-LM, an automated system that allows the latest advances in 3D tissue reconstruction and cellular analysis developments using light microscopy on various tissues, including archived tissue. The workflow in this paper involved advanced tissue sampling methods of the human cerebral cortex, an automated serial section collection system, digital tissue library, cell detection using convolution neural network, 3D cell reconstruction, and advanced analysis. Our results demonstrated the detailed structure of pyramidal cells (number, volume, diameter, sphericity and orientation) and their 3D spatial organization are arranged in a columnar structure. The pipeline of these combined techniques provides a detailed analysis of tissues and cells in biology and pathology.

AB - Techniques involving three-dimensional (3D) tissue structure reconstruction and analysis provide a better understanding of changes in molecules and function. We have developed AutoCUTS-LM, an automated system that allows the latest advances in 3D tissue reconstruction and cellular analysis developments using light microscopy on various tissues, including archived tissue. The workflow in this paper involved advanced tissue sampling methods of the human cerebral cortex, an automated serial section collection system, digital tissue library, cell detection using convolution neural network, 3D cell reconstruction, and advanced analysis. Our results demonstrated the detailed structure of pyramidal cells (number, volume, diameter, sphericity and orientation) and their 3D spatial organization are arranged in a columnar structure. The pipeline of these combined techniques provides a detailed analysis of tissues and cells in biology and pathology.

UR - http://www.scopus.com/inward/record.url?scp=85114639285&partnerID=8YFLogxK

U2 - 10.1038/s42003-021-02548-6

DO - 10.1038/s42003-021-02548-6

M3 - Journal article

C2 - 34475516

AN - SCOPUS:85114639285

VL - 4

JO - Communications Biology

JF - Communications Biology

SN - 2399-3642

IS - 1

M1 - 1030

ER -

ID: 281711877