A toxin-based approach to neuropeptide and peptide hormone discovery
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A toxin-based approach to neuropeptide and peptide hormone discovery. / Koch, Thomas Lund; Torres, Joshua P.; Baskin, Robert P.; Salcedo, Paula Flórez; Chase, Kevin; Olivera, Baldomero M.; Safavi-Hemami, Helena.
I: Frontiers in Molecular Neuroscience, Bind 16, 1176662, 2023.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - A toxin-based approach to neuropeptide and peptide hormone discovery
AU - Koch, Thomas Lund
AU - Torres, Joshua P.
AU - Baskin, Robert P.
AU - Salcedo, Paula Flórez
AU - Chase, Kevin
AU - Olivera, Baldomero M.
AU - Safavi-Hemami, Helena
N1 - Publisher Copyright: Copyright © 2023 Koch, Torres, Baskin, Salcedo, Chase, Olivera and Safavi-Hemami.
PY - 2023
Y1 - 2023
N2 - Peptide hormones and neuropeptides form a diverse class of bioactive secreted molecules that control essential processes in animals. Despite breakthroughs in peptide discovery, many signaling peptides remain undiscovered. Recently, we demonstrated the use of somatostatin-mimicking toxins from cone snails to identify the invertebrate ortholog of somatostatin. Here, we show that this toxin-based approach can be systematically applied to discover other unknown secretory peptides that are likely to have signaling function. Using large sequencing datasets, we searched for homologies between cone snail toxins and secreted proteins from the snails’ prey. We identified and confirmed expression of five toxin families that share strong similarities with unknown secretory peptides from mollusks and annelids and in one case also from ecdysozoans. Based on several lines of evidence we propose that these peptides likely act as signaling peptides that serve important physiological functions. Indeed, we confirmed that one of the identified peptides belongs to the family of crustacean hyperglycemic hormone, a peptide not previously observed in Spiralia. We propose that this discovery pipeline can be broadly applied to other systems in which one organism has evolved molecules to manipulate the physiology of another.
AB - Peptide hormones and neuropeptides form a diverse class of bioactive secreted molecules that control essential processes in animals. Despite breakthroughs in peptide discovery, many signaling peptides remain undiscovered. Recently, we demonstrated the use of somatostatin-mimicking toxins from cone snails to identify the invertebrate ortholog of somatostatin. Here, we show that this toxin-based approach can be systematically applied to discover other unknown secretory peptides that are likely to have signaling function. Using large sequencing datasets, we searched for homologies between cone snail toxins and secreted proteins from the snails’ prey. We identified and confirmed expression of five toxin families that share strong similarities with unknown secretory peptides from mollusks and annelids and in one case also from ecdysozoans. Based on several lines of evidence we propose that these peptides likely act as signaling peptides that serve important physiological functions. Indeed, we confirmed that one of the identified peptides belongs to the family of crustacean hyperglycemic hormone, a peptide not previously observed in Spiralia. We propose that this discovery pipeline can be broadly applied to other systems in which one organism has evolved molecules to manipulate the physiology of another.
KW - Conus
KW - crustacean hyperglycemic hormone
KW - hormone
KW - neuropeptide
KW - toxins
KW - venom
UR - http://www.scopus.com/inward/record.url?scp=85171175342&partnerID=8YFLogxK
U2 - 10.3389/fnmol.2023.1176662
DO - 10.3389/fnmol.2023.1176662
M3 - Journal article
AN - SCOPUS:85171175342
VL - 16
JO - Frontiers in Molecular Neuroscience
JF - Frontiers in Molecular Neuroscience
SN - 1662-5099
M1 - 1176662
ER -
ID: 370493982