Expression of lec-1, a mycobiont gene encoding a galectin-like protein in the lichen Peltigera membranacea

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Standard

Expression of lec-1, a mycobiont gene encoding a galectin-like protein in the lichen Peltigera membranacea. / Miao, Vivian P.W.; Manoharan, Sheeba S.; Snæbjarnarson, Vésteinn; Andrésson, Ólafur S.

I: Symbiosis, Bind 57, Nr. 1, 05.2012, s. 23-31.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Harvard

Miao, VPW, Manoharan, SS, Snæbjarnarson, V & Andrésson, ÓS 2012, 'Expression of lec-1, a mycobiont gene encoding a galectin-like protein in the lichen Peltigera membranacea', Symbiosis, bind 57, nr. 1, s. 23-31. https://doi.org/10.1007/s13199-012-0175-1

APA

Miao, V. P. W., Manoharan, S. S., Snæbjarnarson, V., & Andrésson, Ó. S. (2012). Expression of lec-1, a mycobiont gene encoding a galectin-like protein in the lichen Peltigera membranacea. Symbiosis, 57(1), 23-31. https://doi.org/10.1007/s13199-012-0175-1

Vancouver

Miao VPW, Manoharan SS, Snæbjarnarson V, Andrésson ÓS. Expression of lec-1, a mycobiont gene encoding a galectin-like protein in the lichen Peltigera membranacea. Symbiosis. 2012 maj;57(1):23-31. https://doi.org/10.1007/s13199-012-0175-1

Author

Miao, Vivian P.W. ; Manoharan, Sheeba S. ; Snæbjarnarson, Vésteinn ; Andrésson, Ólafur S. / Expression of lec-1, a mycobiont gene encoding a galectin-like protein in the lichen Peltigera membranacea. I: Symbiosis. 2012 ; Bind 57, Nr. 1. s. 23-31.

Bibtex

@article{2f08a81d766d40e5b9d618e16a0aa2ec,
title = "Expression of lec-1, a mycobiont gene encoding a galectin-like protein in the lichen Peltigera membranacea",
abstract = "The lichen Peltigera membranacea is an association of two symbionts: the mycobiont, a heterotrophic ascomycete, and the photobiont, a Nostoc sp. cyanobacterium. The lec-1 gene, encoding a galectin-like protein, was identified in the metagenome sequence of the lichen, and analysis of the gene structure indicated that it was derived from the mycobiont. This was confirmed by transcriptomic analysis of freshly collected samples in which expression of lec-1 was detected in apothecia and rhizines, tissues that are composed of only fungal cells. Expression of lec-1 in portions of the lichen thallus that included both symbionts was up to two orders of magnitude greater than in tissues lacking photobionts; in contrast, expression of a housekeeping gene stayed relatively constant. These results show that lec-1 expression is influenced by the presence of the photobiont, consistent with a model in which recognition of photobiont ligands by lectins such as lec-1 is a factor in the establishment or maintenance of compatible symbionts.",
keywords = "gpd-1, lec-1, Lectin, Mycobiont, Symbiosis, Transcription, tub-2",
author = "Miao, {Vivian P.W.} and Manoharan, {Sheeba S.} and V{\'e}steinn Sn{\ae}bjarnarson and Andr{\'e}sson, {{\'O}lafur S.}",
year = "2012",
month = may,
doi = "10.1007/s13199-012-0175-1",
language = "English",
volume = "57",
pages = "23--31",
journal = "Symbiosis",
issn = "0334-5114",
publisher = "Springer",
number = "1",

}

RIS

TY - JOUR

T1 - Expression of lec-1, a mycobiont gene encoding a galectin-like protein in the lichen Peltigera membranacea

AU - Miao, Vivian P.W.

AU - Manoharan, Sheeba S.

AU - Snæbjarnarson, Vésteinn

AU - Andrésson, Ólafur S.

PY - 2012/5

Y1 - 2012/5

N2 - The lichen Peltigera membranacea is an association of two symbionts: the mycobiont, a heterotrophic ascomycete, and the photobiont, a Nostoc sp. cyanobacterium. The lec-1 gene, encoding a galectin-like protein, was identified in the metagenome sequence of the lichen, and analysis of the gene structure indicated that it was derived from the mycobiont. This was confirmed by transcriptomic analysis of freshly collected samples in which expression of lec-1 was detected in apothecia and rhizines, tissues that are composed of only fungal cells. Expression of lec-1 in portions of the lichen thallus that included both symbionts was up to two orders of magnitude greater than in tissues lacking photobionts; in contrast, expression of a housekeeping gene stayed relatively constant. These results show that lec-1 expression is influenced by the presence of the photobiont, consistent with a model in which recognition of photobiont ligands by lectins such as lec-1 is a factor in the establishment or maintenance of compatible symbionts.

AB - The lichen Peltigera membranacea is an association of two symbionts: the mycobiont, a heterotrophic ascomycete, and the photobiont, a Nostoc sp. cyanobacterium. The lec-1 gene, encoding a galectin-like protein, was identified in the metagenome sequence of the lichen, and analysis of the gene structure indicated that it was derived from the mycobiont. This was confirmed by transcriptomic analysis of freshly collected samples in which expression of lec-1 was detected in apothecia and rhizines, tissues that are composed of only fungal cells. Expression of lec-1 in portions of the lichen thallus that included both symbionts was up to two orders of magnitude greater than in tissues lacking photobionts; in contrast, expression of a housekeeping gene stayed relatively constant. These results show that lec-1 expression is influenced by the presence of the photobiont, consistent with a model in which recognition of photobiont ligands by lectins such as lec-1 is a factor in the establishment or maintenance of compatible symbionts.

KW - gpd-1

KW - lec-1

KW - Lectin

KW - Mycobiont

KW - Symbiosis

KW - Transcription

KW - tub-2

U2 - 10.1007/s13199-012-0175-1

DO - 10.1007/s13199-012-0175-1

M3 - Journal article

AN - SCOPUS:84865487459

VL - 57

SP - 23

EP - 31

JO - Symbiosis

JF - Symbiosis

SN - 0334-5114

IS - 1

ER -

ID: 371185140