Vision in the small: Reconstructing the structure of protein macromolecules from Cryo-Electron Micrographs

Publikation: Konferencebidrag › Paper › Forskning › fagfællebedømt

Standard

Vision in the small : Reconstructing the structure of protein macromolecules from Cryo-Electron Micrographs. / Mallick, Satya; Agarwal, Sameer; Kriegman, David; Belongie, Serge; Carragher, Bridget; Potter, Clinton.

2006. 1-6 Paper præsenteret ved 2006 17th British Machine Vision Conference, BMVC 2006, Edinburgh, Storbritannien.

Publikation: Konferencebidrag › Paper › Forskning › fagfællebedømt

Harvard

Mallick, S, Agarwal, S, Kriegman, D, Belongie, S, Carragher, B & Potter, C 2006, 'Vision in the small: Reconstructing the structure of protein macromolecules from Cryo-Electron Micrographs', Paper fremlagt ved 2006 17th British Machine Vision Conference, BMVC 2006, Edinburgh, Storbritannien, 04/09/2006 - 07/09/2006 s. 1-6.

APA

Mallick, S., Agarwal, S., Kriegman, D., Belongie, S., Carragher, B., & Potter, C. (2006). Vision in the small: Reconstructing the structure of protein macromolecules from Cryo-Electron Micrographs. 1-6. Paper præsenteret ved 2006 17th British Machine Vision Conference, BMVC 2006, Edinburgh, Storbritannien.

Vancouver

Mallick S, Agarwal S, Kriegman D, Belongie S, Carragher B, Potter C. Vision in the small: Reconstructing the structure of protein macromolecules from Cryo-Electron Micrographs. 2006. Paper præsenteret ved 2006 17th British Machine Vision Conference, BMVC 2006, Edinburgh, Storbritannien.

Author

Mallick, Satya ; Agarwal, Sameer ; Kriegman, David ; Belongie, Serge ; Carragher, Bridget ; Potter, Clinton. / Vision in the small : Reconstructing the structure of protein macromolecules from Cryo-Electron Micrographs. Paper præsenteret ved 2006 17th British Machine Vision Conference, BMVC 2006, Edinburgh, Storbritannien.6 s.

Bibtex

@conference{b3d61a4be2694880a2ffc5d4af3ca878,
title = "Vision in the small: Reconstructing the structure of protein macromolecules from Cryo-Electron Micrographs",
abstract = "Single particle reconstruction using Cryo-Electron Microscopy (cryo-EM) is an emerging technique in structural biology for estimating the 3-D structure (density) of protein macromolecules. Unlike tomography where a large number of images of a specimen can be acquired, the number of images of an individual particle is limited because of radiation damage. Instead, the specimen consists of identical copies of the same protein macro-molecule embedded in vitreous ice at random and unknown 3-D orientations. Because the images are extremely noisy, thousands to hundreds-of-thousands of projections are needed to achieve the desired resolution of 5{\AA}. Along with differences of the imaging modality compared to photographs, single particle reconstruction provides a unique set of challenges to existing computer vision algorithms. Here, we introduce the challenge and opportunity of reconstruction from transmission electron micrographs, and briefly describe our contributions in areas of particle detection, contrast transfer function (CTF) estimation, and initial 3-D model construction.",
author = "Satya Mallick and Sameer Agarwal and David Kriegman and Serge Belongie and Bridget Carragher and Clinton Potter",
year = "2006",
language = "English",
pages = "1--6",
note = "2006 17th British Machine Vision Conference, BMVC 2006 ; Conference date: 04-09-2006 Through 07-09-2006",

}

RIS

TY - CONF

T1 - Vision in the small

T2 - 2006 17th British Machine Vision Conference, BMVC 2006

AU - Mallick, Satya

AU - Agarwal, Sameer

AU - Kriegman, David

AU - Belongie, Serge

AU - Carragher, Bridget

AU - Potter, Clinton

PY - 2006

Y1 - 2006

N2 - Single particle reconstruction using Cryo-Electron Microscopy (cryo-EM) is an emerging technique in structural biology for estimating the 3-D structure (density) of protein macromolecules. Unlike tomography where a large number of images of a specimen can be acquired, the number of images of an individual particle is limited because of radiation damage. Instead, the specimen consists of identical copies of the same protein macro-molecule embedded in vitreous ice at random and unknown 3-D orientations. Because the images are extremely noisy, thousands to hundreds-of-thousands of projections are needed to achieve the desired resolution of 5Å. Along with differences of the imaging modality compared to photographs, single particle reconstruction provides a unique set of challenges to existing computer vision algorithms. Here, we introduce the challenge and opportunity of reconstruction from transmission electron micrographs, and briefly describe our contributions in areas of particle detection, contrast transfer function (CTF) estimation, and initial 3-D model construction.

AB - Single particle reconstruction using Cryo-Electron Microscopy (cryo-EM) is an emerging technique in structural biology for estimating the 3-D structure (density) of protein macromolecules. Unlike tomography where a large number of images of a specimen can be acquired, the number of images of an individual particle is limited because of radiation damage. Instead, the specimen consists of identical copies of the same protein macro-molecule embedded in vitreous ice at random and unknown 3-D orientations. Because the images are extremely noisy, thousands to hundreds-of-thousands of projections are needed to achieve the desired resolution of 5Å. Along with differences of the imaging modality compared to photographs, single particle reconstruction provides a unique set of challenges to existing computer vision algorithms. Here, we introduce the challenge and opportunity of reconstruction from transmission electron micrographs, and briefly describe our contributions in areas of particle detection, contrast transfer function (CTF) estimation, and initial 3-D model construction.

UR - http://www.scopus.com/inward/record.url?scp=84898050721&partnerID=8YFLogxK

M3 - Paper

AN - SCOPUS:84898050721

SP - 1

EP - 6

Y2 - 4 September 2006 through 7 September 2006

ER -

ID: 302053103