Retinal pigment epithelial cells upregulate expression of complement factors after co-culture with activated T cells
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Retinal pigment epithelial cells upregulate expression of complement factors after co-culture with activated T cells. / Juel, Helene Bæk; Kaestel, Charlotte; Folkersen, Lasse; Faber, Carsten; Heegaard, Niels Henrik Helweg; Borup, Rehannah ; Nissen, Mogens Holst.
I: Experimental Eye Research, Bind 92, Nr. 3, 2011, s. 180-88.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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T1 - Retinal pigment epithelial cells upregulate expression of complement factors after co-culture with activated T cells
AU - Juel, Helene Bæk
AU - Kaestel, Charlotte
AU - Folkersen, Lasse
AU - Faber, Carsten
AU - Heegaard, Niels Henrik Helweg
AU - Borup, Rehannah
AU - Nissen, Mogens Holst
PY - 2011
Y1 - 2011
N2 - In this study we examined the effect of T cell-derived cytokines on retinal pigment epithelial (RPE) cells with respect to expression of complement components. We used an in vitro co-culture system in which CD3/CD28-activated human T cells were separated from the human RPE cell line (ARPE-19) by a membrane. Differential gene expression in the RPE cells of complement factor genes was identified using gene arrays, and selected gene transcripts were validated by q-RT-PCR. Protein expression was determined by ELISA and immunoblotting. Co-culture with activated T cells increased RPE mRNA and/or protein expression of complement components C3, factors B, H, H-like 1, CD46, CD55, CD59, and clusterin, in a dose-dependent manner. Soluble factors derived from activated T cells are capable of increasing expression of complement components in RPE cells. This is important for the further understanding of inflammatory ocular diseases such as uveitis and age-related macular degeneration. --------------------------------------------------------------------------------
AB - In this study we examined the effect of T cell-derived cytokines on retinal pigment epithelial (RPE) cells with respect to expression of complement components. We used an in vitro co-culture system in which CD3/CD28-activated human T cells were separated from the human RPE cell line (ARPE-19) by a membrane. Differential gene expression in the RPE cells of complement factor genes was identified using gene arrays, and selected gene transcripts were validated by q-RT-PCR. Protein expression was determined by ELISA and immunoblotting. Co-culture with activated T cells increased RPE mRNA and/or protein expression of complement components C3, factors B, H, H-like 1, CD46, CD55, CD59, and clusterin, in a dose-dependent manner. Soluble factors derived from activated T cells are capable of increasing expression of complement components in RPE cells. This is important for the further understanding of inflammatory ocular diseases such as uveitis and age-related macular degeneration. --------------------------------------------------------------------------------
KW - Faculty of Health and Medical Sciences
KW - RPE
KW - komplement
KW - T celler
U2 - 10.1016/j.exer.2011.01.003
DO - 10.1016/j.exer.2011.01.003
M3 - Journal article
C2 - 21255569
VL - 92
SP - 180
EP - 188
JO - Experimental Eye Research
JF - Experimental Eye Research
SN - 0014-4835
IS - 3
ER -
ID: 38304547