A Practical Approach to Estimating the Effect of Synchrotron Radiation on Sperm Motility and Viability

Publikation: Working paper › Preprint › Forskning

Standard

A Practical Approach to Estimating the Effect of Synchrotron Radiation on Sperm Motility and Viability. / Lindhøj, Mette Bjerg; Bonnin, Anne; Almstrup, Kristian; Dyrby, Tim; Mokso, Rajmund; Sporring, Jon.

bioRxiv, 2023.

Publikation: Working paper › Preprint › Forskning

Harvard

Lindhøj, MB, Bonnin, A, Almstrup, K, Dyrby, T, Mokso, R & Sporring, J 2023 'A Practical Approach to Estimating the Effect of Synchrotron Radiation on Sperm Motility and Viability' bioRxiv. https://doi.org/10.1101/2023.06.08.544169

APA

Lindhøj, M. B., Bonnin, A., Almstrup, K., Dyrby, T., Mokso, R., & Sporring, J. (2023). A Practical Approach to Estimating the Effect of Synchrotron Radiation on Sperm Motility and Viability. bioRxiv. https://doi.org/10.1101/2023.06.08.544169

Vancouver

Lindhøj MB, Bonnin A, Almstrup K, Dyrby T, Mokso R, Sporring J. A Practical Approach to Estimating the Effect of Synchrotron Radiation on Sperm Motility and Viability. bioRxiv. 2023. https://doi.org/10.1101/2023.06.08.544169

Author

Lindhøj, Mette Bjerg ; Bonnin, Anne ; Almstrup, Kristian ; Dyrby, Tim ; Mokso, Rajmund ; Sporring, Jon. / A Practical Approach to Estimating the Effect of Synchrotron Radiation on Sperm Motility and Viability. bioRxiv, 2023.

Bibtex

@techreport{7f75b23b277549d2a92610f9a76174a8,
title = "A Practical Approach to Estimating the Effect of Synchrotron Radiation on Sperm Motility and Viability",
abstract = "Studying the dynamics of sperm tail beating in 3D is important for understanding decreasing fertility trends related to motility. Synchrotron X-ray tomography (SXRT) shows promising results for imaging live biological samples in 3D and time, making it a candidate for imaging sperm tail beating patterns. However, the dose of ionizing radiation (IR) that the cells would receive during image acquisition is of concern as this is likely to affect essential cellular functions. The effect of IR on motility at the dose rates encountered in a synchrotron has never been assessed. Here, results are presented of analyzing the movement and viability of sperm cells exposed at varying durations at the TOMCAT beamline ( Swiss Light Source (SLS)) which has the field of view and spatial resolution needed to reconstruct sperm cells coupled with sub-second exposure times. The results indicate that motility is affected long before viability and that sperm cell dynamics are affected very quickly by the beam.",
author = "Lindh{\o}j, {Mette Bjerg} and Anne Bonnin and Kristian Almstrup and Tim Dyrby and Rajmund Mokso and Jon Sporring",
year = "2023",
doi = "10.1101/2023.06.08.544169",
language = "English",
publisher = "bioRxiv",
type = "WorkingPaper",
institution = "bioRxiv",

}

RIS

TY - UNPB

T1 - A Practical Approach to Estimating the Effect of Synchrotron Radiation on Sperm Motility and Viability

AU - Lindhøj, Mette Bjerg

AU - Bonnin, Anne

AU - Almstrup, Kristian

AU - Dyrby, Tim

AU - Mokso, Rajmund

AU - Sporring, Jon

PY - 2023

Y1 - 2023

N2 - Studying the dynamics of sperm tail beating in 3D is important for understanding decreasing fertility trends related to motility. Synchrotron X-ray tomography (SXRT) shows promising results for imaging live biological samples in 3D and time, making it a candidate for imaging sperm tail beating patterns. However, the dose of ionizing radiation (IR) that the cells would receive during image acquisition is of concern as this is likely to affect essential cellular functions. The effect of IR on motility at the dose rates encountered in a synchrotron has never been assessed. Here, results are presented of analyzing the movement and viability of sperm cells exposed at varying durations at the TOMCAT beamline ( Swiss Light Source (SLS)) which has the field of view and spatial resolution needed to reconstruct sperm cells coupled with sub-second exposure times. The results indicate that motility is affected long before viability and that sperm cell dynamics are affected very quickly by the beam.

AB - Studying the dynamics of sperm tail beating in 3D is important for understanding decreasing fertility trends related to motility. Synchrotron X-ray tomography (SXRT) shows promising results for imaging live biological samples in 3D and time, making it a candidate for imaging sperm tail beating patterns. However, the dose of ionizing radiation (IR) that the cells would receive during image acquisition is of concern as this is likely to affect essential cellular functions. The effect of IR on motility at the dose rates encountered in a synchrotron has never been assessed. Here, results are presented of analyzing the movement and viability of sperm cells exposed at varying durations at the TOMCAT beamline ( Swiss Light Source (SLS)) which has the field of view and spatial resolution needed to reconstruct sperm cells coupled with sub-second exposure times. The results indicate that motility is affected long before viability and that sperm cell dynamics are affected very quickly by the beam.

U2 - 10.1101/2023.06.08.544169

DO - 10.1101/2023.06.08.544169

M3 - Preprint

BT - A Practical Approach to Estimating the Effect of Synchrotron Radiation on Sperm Motility and Viability

PB - bioRxiv

ER -

ID: 375563329