High-throughput verification of transcriptional starting sites by Deep-RACE

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Standard

High-throughput verification of transcriptional starting sites by Deep-RACE. / Olivarius, Signe; Plessy, Charles; Carninci, Piero.

I: BioTechniques, Bind 46, Nr. 2, 2009, s. 130-132.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Harvard

Olivarius, S, Plessy, C & Carninci, P 2009, 'High-throughput verification of transcriptional starting sites by Deep-RACE', BioTechniques, bind 46, nr. 2, s. 130-132. https://doi.org/10.2144/000113066

APA

Olivarius, S., Plessy, C., & Carninci, P. (2009). High-throughput verification of transcriptional starting sites by Deep-RACE. BioTechniques, 46(2), 130-132. https://doi.org/10.2144/000113066

Vancouver

Olivarius S, Plessy C, Carninci P. High-throughput verification of transcriptional starting sites by Deep-RACE. BioTechniques. 2009;46(2):130-132. https://doi.org/10.2144/000113066

Author

Olivarius, Signe ; Plessy, Charles ; Carninci, Piero. / High-throughput verification of transcriptional starting sites by Deep-RACE. I: BioTechniques. 2009 ; Bind 46, Nr. 2. s. 130-132.

Bibtex

@article{104b52d01a7211df8ed1000ea68e967b,
title = "High-throughput verification of transcriptional starting sites by Deep-RACE",
abstract = "We present a high-throughput method for investigating the transcriptional starting sites of genes of interest, which we named Deep-RACE (Deep–rapid amplification of cDNA ends). Taking advantage of the latest sequencing technology, it allows the parallel analysis of multiple genes and is free of time-consuming cloning steps. In comparison to the sequencing of RACE PCR products, our approach is more precise and more cost-effective even for batches as small as 17.",
keywords = "Faculty of Science, promoters, transcription start sites, high throughput, RACE, short-reads sequencing",
author = "Signe Olivarius and Charles Plessy and Piero Carninci",
note = "Paper id:: 19317658",
year = "2009",
doi = "10.2144/000113066",
language = "English",
volume = "46",
pages = "130--132",
journal = "BioTechniques",
issn = "0736-6205",
publisher = "Informa Healthcare",
number = "2",

}

RIS

TY - JOUR

T1 - High-throughput verification of transcriptional starting sites by Deep-RACE

AU - Olivarius, Signe

AU - Plessy, Charles

AU - Carninci, Piero

N1 - Paper id:: 19317658

PY - 2009

Y1 - 2009

N2 - We present a high-throughput method for investigating the transcriptional starting sites of genes of interest, which we named Deep-RACE (Deep–rapid amplification of cDNA ends). Taking advantage of the latest sequencing technology, it allows the parallel analysis of multiple genes and is free of time-consuming cloning steps. In comparison to the sequencing of RACE PCR products, our approach is more precise and more cost-effective even for batches as small as 17.

AB - We present a high-throughput method for investigating the transcriptional starting sites of genes of interest, which we named Deep-RACE (Deep–rapid amplification of cDNA ends). Taking advantage of the latest sequencing technology, it allows the parallel analysis of multiple genes and is free of time-consuming cloning steps. In comparison to the sequencing of RACE PCR products, our approach is more precise and more cost-effective even for batches as small as 17.

KW - Faculty of Science

KW - promoters

KW - transcription start sites

KW - high throughput

KW - RACE

KW - short-reads sequencing

U2 - 10.2144/000113066

DO - 10.2144/000113066

M3 - Journal article

C2 - 19317658

VL - 46

SP - 130

EP - 132

JO - BioTechniques

JF - BioTechniques

SN - 0736-6205

IS - 2

ER -

ID: 17880276