Using hybrid organic-inorganic surface technology to mitigate analyte interactions with metal surfaces in UHPLC
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Using hybrid organic-inorganic surface technology to mitigate analyte interactions with metal surfaces in UHPLC. / Delano, Mathew; Walter, Thomas H; Lauber, Matthew A; Gilar, Martin; Jung, Moon Chul; Nguyen, Jennifer Marie; Boissel, Cheryl; Patel, Amit V; Bates-Harrison, Andrew; Wyndham, Kevin D.
I: Analytical Chemistry, Bind 03, Nr. 14, 2021, s. 5773-5781.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - Using hybrid organic-inorganic surface technology to mitigate analyte interactions with metal surfaces in UHPLC
AU - Delano, Mathew
AU - Walter, Thomas H
AU - Lauber, Matthew A
AU - Gilar, Martin
AU - Jung, Moon Chul
AU - Nguyen, Jennifer Marie
AU - Boissel, Cheryl
AU - Patel, Amit V
AU - Bates-Harrison, Andrew
AU - Wyndham, Kevin D
N1 - Publisher Copyright: © 2021 The Authors. Published by American Chemical Society.
PY - 2021
Y1 - 2021
N2 - Interactions of analytes with metal surfaces in high-performance liquid chromatography (HPLC) instruments and columns have been reported to cause deleterious effects ranging from peak tailing to a complete loss of the analyte signal. These effects are due to the adsorption of certain analytes on the metal oxide layer on the surface of the metal components. We have developed a novel surface modification technology and applied it to the metal components in ultra-HPLC (UHPLC) instruments and columns to mitigate these interactions. A hybrid organic-inorganic surface, based on an ethylene-bridged siloxane chemistry, was developed for use with reversed-phase and hydrophilic interaction chromatography. We have characterized the performance of UHPLC instruments and columns that incorporate this surface technology and compared the results with those obtained using their conventional counterparts. We demonstrate improved performance when using the hybrid surface technology for separations of nucleotides, a phosphopeptide, and an oligonucleotide. The hybrid surface technology was found to result in higher and more consistent analyte peak areas and improved peak shape, particularly when using low analyte mass loads and acidic mobile phases. Reduced abundances of iron adducts in the mass spectrum of a peptide were also observed when using UHPLC systems and columns that incorporate hybrid surface technology. These results suggest that this technology will be particularly beneficial in UHPLC/mass spectrometry investigations of metal-sensitive analytes.
AB - Interactions of analytes with metal surfaces in high-performance liquid chromatography (HPLC) instruments and columns have been reported to cause deleterious effects ranging from peak tailing to a complete loss of the analyte signal. These effects are due to the adsorption of certain analytes on the metal oxide layer on the surface of the metal components. We have developed a novel surface modification technology and applied it to the metal components in ultra-HPLC (UHPLC) instruments and columns to mitigate these interactions. A hybrid organic-inorganic surface, based on an ethylene-bridged siloxane chemistry, was developed for use with reversed-phase and hydrophilic interaction chromatography. We have characterized the performance of UHPLC instruments and columns that incorporate this surface technology and compared the results with those obtained using their conventional counterparts. We demonstrate improved performance when using the hybrid surface technology for separations of nucleotides, a phosphopeptide, and an oligonucleotide. The hybrid surface technology was found to result in higher and more consistent analyte peak areas and improved peak shape, particularly when using low analyte mass loads and acidic mobile phases. Reduced abundances of iron adducts in the mass spectrum of a peptide were also observed when using UHPLC systems and columns that incorporate hybrid surface technology. These results suggest that this technology will be particularly beneficial in UHPLC/mass spectrometry investigations of metal-sensitive analytes.
KW - Faculty of Science
KW - Iron
KW - Peptides and proteins
KW - Metals
KW - Chromatography
KW - Biopolymers
UR - http://www.scopus.com/inward/record.url?scp=85104460849&partnerID=8YFLogxK
U2 - 10.1021/acs.analchem.0c05203
DO - 10.1021/acs.analchem.0c05203
M3 - Journal article
C2 - 33798331
AN - SCOPUS:85104460849
VL - 03
SP - 5773
EP - 5781
JO - Industrial And Engineering Chemistry Analytical Edition
JF - Industrial And Engineering Chemistry Analytical Edition
SN - 0003-2700
IS - 14
ER -
ID: 262743555