Fast phylogenetic DNA barcoding

Research output: Contribution to journalJournal articleResearchpeer-review

We present a heuristic approach to the DNA assignment problem based on phylogenetic inferences using constrained neighbour joining and non-parametric bootstrapping. We show that this method performs as well as the more computationally intensive full Bayesian approach in an analysis of 500 insect DNA sequences obtained from GenBank. We also analyse a previously published dataset of environmental DNA sequences from soil from New Zealand and Siberia, and use these data to illustrate the fact that statistical approaches to the DNA assignment problem allow for more appropriate criteria for determining the taxonomic level at which a particular DNA sequence can be assigned.
Original languageEnglish
JournalPhilosophical Transactions of the Royal Society of London. Biological Sciences
Issue number1512
Pages (from-to)3997-4002
Number of pages6
Publication statusPublished - 2008

Bibliographical note

KEYWORDS: assignment, barcoding, phylogenetics, neighbour joining

ID: 9541977