Susceptibility of bacteria isolated from pigs to tiamulin and enrofloxacin metabolites
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Susceptibility of bacteria isolated from pigs to tiamulin and enrofloxacin metabolites. / Lykkeberg, Anne Kruse; Halling-Sørensen, Bent; Jensen, Lars Bogø.
In: Veterinary Microbiology, Vol. 121, No. 1-2, 2007, p. 116-124.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Susceptibility of bacteria isolated from pigs to tiamulin and enrofloxacin metabolites
AU - Lykkeberg, Anne Kruse
AU - Halling-Sørensen, Bent
AU - Jensen, Lars Bogø
PY - 2007
Y1 - 2007
N2 - Susceptibilities to metabolites of tiamulin (TIA) and enrofloxacin (ENR) were tested using selected bacteria with previously defined minimal inhibitory concentrations (MIC). The TIA metabolites tested were: N-deethyl-tiamulin (DTIA), 2ß-hydroxy-tiamulin (2ß-HTIA) and 8a-hydroxy-tiamulin (8a-HTIA), and the ENR metabolites were: ciprofloxacin (CIP) and enrofloxacin N-oxide (ENR-N). Bacteria, all of porcine origin, were selected as representatives of bacterial infections (Staphylococcus hyicus and Actinobacillus pleuropneumoniae), zoonotic bacteria (Campylobacter coli) and indicator bacteria (Escherichia coli and enterococci). Furthermore the effects of these compounds were tested on the microbial community of active sludge to test any negative effect on colony forming units (CFU). DTIA had a potency of 12.5-50% of the potency of TIA. 2ß-HTIA and 8a-HTIA had potencies less than 1% of the potency of TIA. ENR-N had a potency of 0.75-1.5% of the potency of ENR, while CIP and ENR had similar potencies. Results obtained here indicate that CIP and DTIA could contribute to the selective pressure for upholding antimicrobial resistant bacteria in animals under ENR or TIA treatment. The most potent metabolites CIP and DTIA showed considerable potencies against activated sludge bacteria compared to the parent compounds. EC50 (µg/ml) for ENR, CIP, TIA and DTIA were 0.018 [95% CI: 0.028-0.149], 0.064 [95% CI: 0.007-0.046], 6.0 [95% CI: 3.6-9.8], and 9.7 [95% CI: 5.8-16.3], respectively. This indicates that the compounds can change the bacterial population in the sludge, and hereby alter the properties of the sludge.
AB - Susceptibilities to metabolites of tiamulin (TIA) and enrofloxacin (ENR) were tested using selected bacteria with previously defined minimal inhibitory concentrations (MIC). The TIA metabolites tested were: N-deethyl-tiamulin (DTIA), 2ß-hydroxy-tiamulin (2ß-HTIA) and 8a-hydroxy-tiamulin (8a-HTIA), and the ENR metabolites were: ciprofloxacin (CIP) and enrofloxacin N-oxide (ENR-N). Bacteria, all of porcine origin, were selected as representatives of bacterial infections (Staphylococcus hyicus and Actinobacillus pleuropneumoniae), zoonotic bacteria (Campylobacter coli) and indicator bacteria (Escherichia coli and enterococci). Furthermore the effects of these compounds were tested on the microbial community of active sludge to test any negative effect on colony forming units (CFU). DTIA had a potency of 12.5-50% of the potency of TIA. 2ß-HTIA and 8a-HTIA had potencies less than 1% of the potency of TIA. ENR-N had a potency of 0.75-1.5% of the potency of ENR, while CIP and ENR had similar potencies. Results obtained here indicate that CIP and DTIA could contribute to the selective pressure for upholding antimicrobial resistant bacteria in animals under ENR or TIA treatment. The most potent metabolites CIP and DTIA showed considerable potencies against activated sludge bacteria compared to the parent compounds. EC50 (µg/ml) for ENR, CIP, TIA and DTIA were 0.018 [95% CI: 0.028-0.149], 0.064 [95% CI: 0.007-0.046], 6.0 [95% CI: 3.6-9.8], and 9.7 [95% CI: 5.8-16.3], respectively. This indicates that the compounds can change the bacterial population in the sludge, and hereby alter the properties of the sludge.
KW - Former Faculty of Pharmaceutical Sciences
U2 - 10.1016/j.vetmic.2006.11.020
DO - 10.1016/j.vetmic.2006.11.020
M3 - Journal article
C2 - 17194550
VL - 121
SP - 116
EP - 124
JO - Veterinary Microbiology
JF - Veterinary Microbiology
SN - 0378-1135
IS - 1-2
ER -
ID: 2307113