Enzyme-linked immunosorbent assay (ELISAs) for metalloproteinase derived type II collagen neoepitope, CIIM--increased serum CIIM in subjects with severe radiographic osteoarthritis
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Enzyme-linked immunosorbent assay (ELISAs) for metalloproteinase derived type II collagen neoepitope, CIIM--increased serum CIIM in subjects with severe radiographic osteoarthritis. / Bay-Jensen, Anne-Christine; Liu, Qi; Byrjalsen, Inger; Li, Yi; Wang, Jianxia; Pedersen, Christian; Leeming, Diana J; Dam, Erik B; Zheng, Qinlong; Qvist, Per; Karsdal, Morten A.
In: Clinical Biochemistry, Vol. 44, No. 5-6, 04.2011, p. 423-9.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Enzyme-linked immunosorbent assay (ELISAs) for metalloproteinase derived type II collagen neoepitope, CIIM--increased serum CIIM in subjects with severe radiographic osteoarthritis
AU - Bay-Jensen, Anne-Christine
AU - Liu, Qi
AU - Byrjalsen, Inger
AU - Li, Yi
AU - Wang, Jianxia
AU - Pedersen, Christian
AU - Leeming, Diana J
AU - Dam, Erik B
AU - Zheng, Qinlong
AU - Qvist, Per
AU - Karsdal, Morten A
N1 - Copyright © 2011 The Canadian Society of Clinical Chemists. Published by Elsevier Inc. All rights reserved.
PY - 2011/4
Y1 - 2011/4
N2 - OBJECTIVES: In joint degenerative diseases, the collagens are degraded by matrix metalloproteinases and protein fragments are released to serum as potential biomarkers.METHODS: A collagen type II specific neoepitope, CIIM, was identified (…RDGAAG(1053)) by mass spectrometry. Two ELISAs against the neoepitope were developed. CIIM was measured in cartilage explants in the presence or absence of protease inhibitors. CIIM was measured in OA synovial fluid (n=51) and serum (n=156). Knee OA was graded by standard Kellgren-Lawrence (KL) score.RESULTS: The ELISAs showed good technical performance; CV%, <13%. CIIM release from cartilage explants was blocked by the MMP inhibitor. CIIM was detected in synovial fluid. Furthermore, serum CIIM levels were significantly higher (P<0.05) in those individuals with mild or severe OA than in those with no OA.CONCLUSION: We developed a new biomarker for joint degenerative diseases, which we demonstrated was derived from MMP-degraded type II collagen.
AB - OBJECTIVES: In joint degenerative diseases, the collagens are degraded by matrix metalloproteinases and protein fragments are released to serum as potential biomarkers.METHODS: A collagen type II specific neoepitope, CIIM, was identified (…RDGAAG(1053)) by mass spectrometry. Two ELISAs against the neoepitope were developed. CIIM was measured in cartilage explants in the presence or absence of protease inhibitors. CIIM was measured in OA synovial fluid (n=51) and serum (n=156). Knee OA was graded by standard Kellgren-Lawrence (KL) score.RESULTS: The ELISAs showed good technical performance; CV%, <13%. CIIM release from cartilage explants was blocked by the MMP inhibitor. CIIM was detected in synovial fluid. Furthermore, serum CIIM levels were significantly higher (P<0.05) in those individuals with mild or severe OA than in those with no OA.CONCLUSION: We developed a new biomarker for joint degenerative diseases, which we demonstrated was derived from MMP-degraded type II collagen.
KW - Aged
KW - Aged, 80 and over
KW - Collagen Type II
KW - Enzyme-Linked Immunosorbent Assay
KW - Female
KW - Humans
KW - Male
KW - Metalloproteases
KW - Middle Aged
KW - Osteoarthritis
KW - Journal Article
U2 - 10.1016/j.clinbiochem.2011.01.001
DO - 10.1016/j.clinbiochem.2011.01.001
M3 - Journal article
C2 - 21223960
VL - 44
SP - 423
EP - 429
JO - Clinical Biochemistry
JF - Clinical Biochemistry
SN - 0009-9120
IS - 5-6
ER -
ID: 187548692