In vivo study of experimental pneumococcal meningitis using magnetic resonance imaging: BMC Medical Imaging
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In vivo study of experimental pneumococcal meningitis using magnetic resonance imaging : BMC Medical Imaging. / Brandt, C.T.; Simonsen, H.; Liptrot, Matthew George; Søgaard, L.V.; Lundgren, J.D.; Østergaard, C.; Frimodt-Møller, N.; Rowland, I.J.
In: BMC Med. Imaging, Vol. 8, 2008.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - In vivo study of experimental pneumococcal meningitis using magnetic resonance imaging
T2 - BMC Medical Imaging
AU - Brandt, C.T.
AU - Simonsen, H.
AU - Liptrot, Matthew George
AU - Søgaard, L.V.
AU - Lundgren, J.D.
AU - Østergaard, C.
AU - Frimodt-Møller, N.
AU - Rowland, I.J.
PY - 2008
Y1 - 2008
N2 - Background: Magnetic Resonance Imaging (MRI) methods were evaluated as a tool for the study of experimental meningitis. The identification and characterisation of pathophysiological parameters that vary during the course of the disease could be used as markers for future studies of new treatment strategies. Methods: Rats infected intracisternally with S. pneumoniae (n = 29) or saline (n = 13) were randomized for imaging at 6, 12, 24, 30, 36, 42 or 48 hours after infection. T1W, T2W, quantitative diffusion, and post contrast T1W images were acquired at 4.7 T. Dynamic MRI (dMRI) was used to evaluate blood-brain-barrier (BBB) permeability and to obtain a measure of cerebral and muscle perfusion. Clinical- and motor scores, bacterial counts in CSF and blood, and WBC counts in CSF were measured. Results: MR images and dMRI revealed the development of a highly significant increase in BBB permeability (P <0.002) and ventricle size (P <0.0001) among infected rats. Clinical disease severity was closely related to ventricle expansion (P = 0.024). Changes in brain water distribution, assessed by ADC, and categorization of brain 'perfusion' by cortex ΔSI(bolus) were subject to increased inter-rat variation as the disease progressed, but without overall differences compared to uninfected rats (P > 0.05). Areas of well-'perfused' muscle decreased with the progression of infection indicative of septicaemia (P = 0.05). Conclusion: The evolution of bacterial meningitis was successfully followed in-vivo with MRI. Increasing BBB-breakdown and ventricle size was observed in rats with meningitis whereas changes in brain water distribution were heterogeneous. MRI will be a valuable technique for future studies aiming at evaluating or optimizing adjunctive treatments. © 2008 Brandt et al; licensee BioMed Central Ltd.
AB - Background: Magnetic Resonance Imaging (MRI) methods were evaluated as a tool for the study of experimental meningitis. The identification and characterisation of pathophysiological parameters that vary during the course of the disease could be used as markers for future studies of new treatment strategies. Methods: Rats infected intracisternally with S. pneumoniae (n = 29) or saline (n = 13) were randomized for imaging at 6, 12, 24, 30, 36, 42 or 48 hours after infection. T1W, T2W, quantitative diffusion, and post contrast T1W images were acquired at 4.7 T. Dynamic MRI (dMRI) was used to evaluate blood-brain-barrier (BBB) permeability and to obtain a measure of cerebral and muscle perfusion. Clinical- and motor scores, bacterial counts in CSF and blood, and WBC counts in CSF were measured. Results: MR images and dMRI revealed the development of a highly significant increase in BBB permeability (P <0.002) and ventricle size (P <0.0001) among infected rats. Clinical disease severity was closely related to ventricle expansion (P = 0.024). Changes in brain water distribution, assessed by ADC, and categorization of brain 'perfusion' by cortex ΔSI(bolus) were subject to increased inter-rat variation as the disease progressed, but without overall differences compared to uninfected rats (P > 0.05). Areas of well-'perfused' muscle decreased with the progression of infection indicative of septicaemia (P = 0.05). Conclusion: The evolution of bacterial meningitis was successfully followed in-vivo with MRI. Increasing BBB-breakdown and ventricle size was observed in rats with meningitis whereas changes in brain water distribution were heterogeneous. MRI will be a valuable technique for future studies aiming at evaluating or optimizing adjunctive treatments. © 2008 Brandt et al; licensee BioMed Central Ltd.
KW - gadolinium pentetate
KW - animal experiment
KW - animal model
KW - animal tissue
KW - article
KW - bacterial count
KW - bacterial meningitis
KW - blood brain barrier
KW - blood level
KW - blood vessel permeability
KW - brain perfusion
KW - brain ventricle dilatation
KW - brain water
KW - cerebrospinal fluid analysis
KW - contrast enhancement
KW - controlled study
KW - disease course
KW - disease severity
KW - histopathology
KW - in vivo study
KW - leukocyte count
KW - male
KW - motor performance
KW - muscle perfusion
KW - neuropathology
KW - nonhuman
KW - nuclear magnetic resonance imaging
KW - rat
KW - scoring system
KW - septicemia
KW - Streptococcus pneumoniae
KW - animal
KW - brain ventricle
KW - evaluation
KW - image enhancement
KW - methodology
KW - pathology
KW - Wistar rat
KW - Animals
KW - Blood-Brain Barrier
KW - Cerebral Ventricles
KW - Image Enhancement
KW - Magnetic Resonance Imaging
KW - Male
KW - Meningitis, Pneumococcal
KW - Rats
KW - Rats, Wistar
U2 - 10.1186/1471-2342-8-1
DO - 10.1186/1471-2342-8-1
M3 - Journal article
C2 - 18194516
VL - 8
JO - BMC Medical Imaging
JF - BMC Medical Imaging
SN - 1471-2342
ER -
ID: 137009316