Drosophila Imp iCLIP identifies an RNA assemblage coordinating F-actin formation
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Drosophila Imp iCLIP identifies an RNA assemblage coordinating F-actin formation. / Hansen, Heidi Theil; Rasmussen, Simon Horskjær; Adolph, Sidsel Kramshøj; Plass, Mireya; Krogh, Anders; Sanford, Jeremy; Nielsen, Finn Cilius; Christiansen, Jan.
In: Genome Biology (Online Edition), Vol. 16, 123, 2015.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Drosophila Imp iCLIP identifies an RNA assemblage coordinating F-actin formation
AU - Hansen, Heidi Theil
AU - Rasmussen, Simon Horskjær
AU - Adolph, Sidsel Kramshøj
AU - Plass, Mireya
AU - Krogh, Anders
AU - Sanford, Jeremy
AU - Nielsen, Finn Cilius
AU - Christiansen, Jan
PY - 2015
Y1 - 2015
N2 - BACKGROUND: Post-transcriptional RNA regulons ensure co-ordinated expression of monocistronic mRNAs encoding functionally related proteins. In this study, we employ a combination of RIP-seq and short- and long-wave individual-nucleotide resolution crosslinking and immunoprecipitation (iCLIP) technologies in Drosophila cells to identify transcripts associated with cytoplasmic ribonucleoproteins (RNPs) containing the RNA-binding protein Imp.RESULTS: We find extensive binding of Imp to 3'UTRs of transcripts that are involved in F-actin formation. A common denominator of the RNA-protein interface is the presence of multiple motifs with a central UA-rich element flanked by CA-rich elements. Experiments in single cells and intact flies reveal compromised actin cytoskeletal dynamics associated with low Imp levels. The former shows reduced F-actin formation and the latter exhibits abnormal neuronal patterning. This demonstrates a physiological significance of the defined RNA regulon.CONCLUSIONS: Our data imply that Drosophila Imp RNPs may function as cytoplasmic mRNA assemblages that encode proteins which participate in actin cytoskeletal remodeling. Thus, they may facilitate co-ordinated protein expression in sub-cytoplasmic locations such as growth cones.
AB - BACKGROUND: Post-transcriptional RNA regulons ensure co-ordinated expression of monocistronic mRNAs encoding functionally related proteins. In this study, we employ a combination of RIP-seq and short- and long-wave individual-nucleotide resolution crosslinking and immunoprecipitation (iCLIP) technologies in Drosophila cells to identify transcripts associated with cytoplasmic ribonucleoproteins (RNPs) containing the RNA-binding protein Imp.RESULTS: We find extensive binding of Imp to 3'UTRs of transcripts that are involved in F-actin formation. A common denominator of the RNA-protein interface is the presence of multiple motifs with a central UA-rich element flanked by CA-rich elements. Experiments in single cells and intact flies reveal compromised actin cytoskeletal dynamics associated with low Imp levels. The former shows reduced F-actin formation and the latter exhibits abnormal neuronal patterning. This demonstrates a physiological significance of the defined RNA regulon.CONCLUSIONS: Our data imply that Drosophila Imp RNPs may function as cytoplasmic mRNA assemblages that encode proteins which participate in actin cytoskeletal remodeling. Thus, they may facilitate co-ordinated protein expression in sub-cytoplasmic locations such as growth cones.
U2 - 10.1186/s13059-015-0687-0
DO - 10.1186/s13059-015-0687-0
M3 - Journal article
C2 - 26054396
VL - 16
JO - Genome Biology (Online Edition)
JF - Genome Biology (Online Edition)
SN - 1474-7596
M1 - 123
ER -
ID: 139969241