Interactions between PAMAM-NH2 and 6-mercaptopurine: qualitative and quantitative NMR studies

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Interactions between PAMAM-NH2 and 6-mercaptopurine: qualitative and quantitative NMR studies. / Gao, Xueke; Ma, Minjun; Pedersen, Christian Marcus; Liu, Rui; Zhang, Zhenzhou; Chang, Honghong; Qiao, Yan; Wang, Yingxiong.

In: Chemistry - An Asian Journal, Vol. n/a, No. n/a, 08.09.2021, p. 3658-3663.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Gao, X, Ma, M, Pedersen, CM, Liu, R, Zhang, Z, Chang, H, Qiao, Y & Wang, Y 2021, 'Interactions between PAMAM-NH2 and 6-mercaptopurine: qualitative and quantitative NMR studies', Chemistry - An Asian Journal, vol. n/a, no. n/a, pp. 3658-3663. https://doi.org/10.1002/asia.202100771

APA

Gao, X., Ma, M., Pedersen, C. M., Liu, R., Zhang, Z., Chang, H., Qiao, Y., & Wang, Y. (2021). Interactions between PAMAM-NH2 and 6-mercaptopurine: qualitative and quantitative NMR studies. Chemistry - An Asian Journal, n/a(n/a), 3658-3663. https://doi.org/10.1002/asia.202100771

Vancouver

Gao X, Ma M, Pedersen CM, Liu R, Zhang Z, Chang H et al. Interactions between PAMAM-NH2 and 6-mercaptopurine: qualitative and quantitative NMR studies. Chemistry - An Asian Journal. 2021 Sep 8;n/a(n/a):3658-3663. https://doi.org/10.1002/asia.202100771

Author

Gao, Xueke ; Ma, Minjun ; Pedersen, Christian Marcus ; Liu, Rui ; Zhang, Zhenzhou ; Chang, Honghong ; Qiao, Yan ; Wang, Yingxiong. / Interactions between PAMAM-NH2 and 6-mercaptopurine: qualitative and quantitative NMR studies. In: Chemistry - An Asian Journal. 2021 ; Vol. n/a, No. n/a. pp. 3658-3663.

Bibtex

@article{74f121a95094481a98d758cf8d0ab638,
title = "Interactions between PAMAM-NH2 and 6-mercaptopurine: qualitative and quantitative NMR studies",
abstract = "Despite being used as an anti-leukemic drug, the poor solubility of 6-mercaptopurine (6-MP) limits its use in topical and parenteral applications. Dendrimers are commonly used as drug carriers to improve their solubility in aqueous solution. In this work, the interactions between 6-MP and the amine-terminated poly(amidoamine) dendrimers (PAMAM-NH2 ) were investigated by various NMR technology. The chemical shift titrations disclosed that the 6-MP interacted with the surface of PAMAM-NH2 mainly through electrostatics. The determination of diffusion coefficient and relaxation measurements further confirmed the presence of interactions in 6-MP/PAMAM-NH2 complexes. In addition, the encapsulation of 6-MP within the cavity of PAMAM-NH2 was revealed through nuclear Overhauser effect spectroscopy and Saturation Transfer Double Difference analysis. Finally, the binding strength (H-8 is 1002 is 70 of 6-MP to PAMAM-NH2 was quantitatively expressed using epitope maps. This study provides a systematic methodology for qualitative and quantitative studies of the interactions between dendrimers and drug molecules in general.",
keywords = "Faculty of Science, 6-MP, binding strength, interaction, NMR, PAMAM-NH2",
author = "Xueke Gao and Minjun Ma and Pedersen, {Christian Marcus} and Rui Liu and Zhenzhou Zhang and Honghong Chang and Yan Qiao and Yingxiong Wang",
year = "2021",
month = sep,
day = "8",
doi = "10.1002/asia.202100771",
language = "English",
volume = "n/a",
pages = "3658--3663",
journal = "Chemistry - An Asian Journal",
issn = "1861-4728",
publisher = "Wiley - V C H Verlag GmbH & Co. KGaA",
number = "n/a",

}

RIS

TY - JOUR

T1 - Interactions between PAMAM-NH2 and 6-mercaptopurine: qualitative and quantitative NMR studies

AU - Gao, Xueke

AU - Ma, Minjun

AU - Pedersen, Christian Marcus

AU - Liu, Rui

AU - Zhang, Zhenzhou

AU - Chang, Honghong

AU - Qiao, Yan

AU - Wang, Yingxiong

PY - 2021/9/8

Y1 - 2021/9/8

N2 - Despite being used as an anti-leukemic drug, the poor solubility of 6-mercaptopurine (6-MP) limits its use in topical and parenteral applications. Dendrimers are commonly used as drug carriers to improve their solubility in aqueous solution. In this work, the interactions between 6-MP and the amine-terminated poly(amidoamine) dendrimers (PAMAM-NH2 ) were investigated by various NMR technology. The chemical shift titrations disclosed that the 6-MP interacted with the surface of PAMAM-NH2 mainly through electrostatics. The determination of diffusion coefficient and relaxation measurements further confirmed the presence of interactions in 6-MP/PAMAM-NH2 complexes. In addition, the encapsulation of 6-MP within the cavity of PAMAM-NH2 was revealed through nuclear Overhauser effect spectroscopy and Saturation Transfer Double Difference analysis. Finally, the binding strength (H-8 is 1002 is 70 of 6-MP to PAMAM-NH2 was quantitatively expressed using epitope maps. This study provides a systematic methodology for qualitative and quantitative studies of the interactions between dendrimers and drug molecules in general.

AB - Despite being used as an anti-leukemic drug, the poor solubility of 6-mercaptopurine (6-MP) limits its use in topical and parenteral applications. Dendrimers are commonly used as drug carriers to improve their solubility in aqueous solution. In this work, the interactions between 6-MP and the amine-terminated poly(amidoamine) dendrimers (PAMAM-NH2 ) were investigated by various NMR technology. The chemical shift titrations disclosed that the 6-MP interacted with the surface of PAMAM-NH2 mainly through electrostatics. The determination of diffusion coefficient and relaxation measurements further confirmed the presence of interactions in 6-MP/PAMAM-NH2 complexes. In addition, the encapsulation of 6-MP within the cavity of PAMAM-NH2 was revealed through nuclear Overhauser effect spectroscopy and Saturation Transfer Double Difference analysis. Finally, the binding strength (H-8 is 1002 is 70 of 6-MP to PAMAM-NH2 was quantitatively expressed using epitope maps. This study provides a systematic methodology for qualitative and quantitative studies of the interactions between dendrimers and drug molecules in general.

KW - Faculty of Science

KW - 6-MP

KW - binding strength

KW - interaction

KW - NMR

KW - PAMAM-NH2

U2 - 10.1002/asia.202100771

DO - 10.1002/asia.202100771

M3 - Journal article

C2 - 34494362

VL - n/a

SP - 3658

EP - 3663

JO - Chemistry - An Asian Journal

JF - Chemistry - An Asian Journal

SN - 1861-4728

IS - n/a

ER -

ID: 279782632